Lab Demonstrator
– Miss Mehwish Khalid
– Email: [email protected]
– Contact: 05190854288
Introduction:
In order to conduct Microbial analysis of all environmental samples at Institute of Environmental Sciences and Engineering (IESE), Environmental Microbiology Teaching Lab was established in 2005.
Research activities in the Environmental microbiology teaching lab are divided between teaching basic microbiology experiments to the undergrad and providing full state-of-art equipment and lab infra-structure for conducting MS and PhD level research. The sophisticated instrumentation in lab needs proper care and precautionary measures. Keeping this in view all the research students are advised to follow the set of instructions.
Major Tests
- Bacteriological examination of water by MF Technique
- Microbiological Monitoring of Air and water Quality
- Effect of Temperature on Growth of Microorganisms
- Study of the Algal population of waste stabilization ponds
- Enumeration of Soil Microorganisms
- Identification of bacterial species
- Genotoxicity of Chlorine by-products
- Biodegradation studies
- PCR and gene sequencing
- Testing of erythrocyte nuclear abnormalities
- Enumeration of helminthes eggs and parasitic worms
- Translocation pf microorganisms in plants
- Enumeration of entophytes
- Quorum sensing
Available parameters
- Biochemical tests (Oxidase, Catalase)
- “Detection/Isolation/Quantification of
E.coli
Salmonella
Shigella
Bacillus
Pseudomonas” - Electrical Conductivity
- Motility
- Optical Density
- pH
- Total phosphorous
Microbial Water Quality Analysis
The testing is precise, accurate and reliable due to the latest apparatus available in the lab in addition to the expertise of the staff. The entire equipment used in testing is regularly calibrated in order to maintain its accuracy.
- Standard Plate Count Test
- Standard Coliform
- Fecal Coliform
Major Equipment:
Gradient Thermal Cycler
It is used to amplify segments of DNA via the polymerase chain reaction (PCR). Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics
Gel Electrophoresis Horizontal
It is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
Gel Doc system
It is used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.
Digital Autoclave
It is to sterilize equipment, glassware and media by subjecting them to high pressure saturated steam at 121 °C (249°F) for around 15–20 minutes depending on the size of the load and the contents.
Incubator (Adaptive multifunctional Digital PID Capacity) :
It is a heated, insulated box used to grow and maintain microbiological or cell cultures. The incubator maintains optimal temperature, humidity and gaseous content of the atmosphere inside.
Portable UV/Vis Spectrophotometer
Purpose of this is to measure Optical density of a sample. It can measure intensity as a function of the light source wavelength
Laminar Flow Hood
Provide sterilize environment for microbial inoculations and culture handling.
Refrigerated Micro centrifuge:
A microcentrifuge, is used to spin small (2 ml or less) liquid samples at high speeds. Centrifugation of small samples is important for many biological applications, such as pelleting nucleic acids for downstream application such as PCR and sequencing, or isolating proteins from solution for use in western blot or 2D electrophoresis. Refrigerated centrifuges protect sensitive samples from heat degeneration, protect the sample’s integrity.
Ice Flaking Machine:
Ice flaking machine is conceptualized and designed to meet the continuous requirement of ice to store biological samples along with transportation to other facilities. It is made up of corrosion free metal components with advance insulation ensuring maximum thermal efficiency and durability.
Leica Microscope
An instrument used to observe objects that are too small for the naked eye. Light microscopes from Leica Microsystems are of high quality and meet the highest demands whatever the application – from routine laboratory work to the research of multi-dimensional dynamic processes in living cells.
Water Bath
It is a device for regulating the temperature of anything subjected to heat, by surrounding the vessel containing it with another vessel containing water which can be kept at a desired temperature.
DO meter:
Measures dissolved oxygen in wastewater, surface water and aquaculture applications. Calibrates easily in a saturated air environment and displays in % or ppm (mg/L) values.
Colony counter
Petri plates are inserted inside the colony counter where they are properly lit and magnified. Operators can tick detected colonies with a specifically devised marker, meanwhile a digital display will increase the total counts.
Orbital Shaker
It is used to blend or agitate samples within flasks or tubes. These shakers also provide convenience of programmable speed as well as running time with option of customizing its platform as per ones requirement and application.
Gallery:
