Environmental Anaerobic Lab

Lab PI/ Incharge

– Dr. Imran Hashmi

– Email: [email protected]

– Contact:051-90854309

LAB DEMONSTRATOR

– Miss Mehwish Khalid

– Email: [email protected]

– Contact: 05190854288

Introduction:

In 2023, an anaerobic lab was established to conduct microbial and molecular analyses on various environmental samples. This state-of-the-art facility, supported by our collaborators on the MRC UK-funded project titled “Quantifying the Transmission Routes of Gastroenteritis in Pakistan and Developing Targeted Interventions," is equipped with advanced instrumentation to meet the rigorous demands of molecular and microbial research. The lab’s capabilities are integral to advancing our understanding of pathogen transmission and developing effective intervention strategies.

Major Tests

• Culturing of anaerobic bacteria in controlled chamber
• Identification of bacterial species
• Handling of water, sediment and fecal (poultry and human) samples
• Handling of clinical samples in biosafety cabinets for culturing pathogenic bacteria
• DNA extraction from environmental and fecal samples
• Quantification and identification of bacterial species through qPCR analysis
• Preparation of plate sweep samples for metagenomic analysis
• Antimicrobial resistance through disc diffusion method